The thick shelled river mussel (Unio crassus) has a fragmented distribution in southern Sweden. It is a threatened and protected species. In both the Swedish red list and the IUCN Red List of Threatened Species Unio crassus is classified as Endangered (EN). Causes for this classification are for instance differentiated bottom substrate and deterioration in water quality. In this study, habitat preferences of Unio crassus were investigated to assist in future restoration work.

The wood-living beetle Ceruchus chrysomelinus is a rare species connected to natural forests. In Sweden, it is red-listed as endangered (EN) and an action plan for its preservation has been done by the Swedish Environmental Protection Agency. In this work I have therefore investigated how a translocation of the species succeeded after 17 years. I aimed to study the species dispersal biology, its substrate preferences and the supply of suitable wood on this site. The study was performed during 2012 in Pansaruddens nature reserve close to Uppsala in Sweden, which is a spruce dominated forest.

Disturbances, such as fire and wind, are important for saproxylic beetles (= beetles depending on decaying wood) to gain substrate in boreal forests. Clear-cutting is an example of a man-made disturbance. Measures such as prescribed burning have been made to resemble natural disturbances. The aim of this study was to see which clear-cut characteristics are important for the occurrence of the saproxylic beetle Upis ceramboides. This is a species favored by open habitats and is said to respond positively to forest fires. The distribution area in Sweden for this species has decreased during the last two centuries and I wanted to see if there were differences between clear-cuts in Hälsingland, where it is very rare and decreasing, and Norrbotten where this study was conducted.

In this thesis the methane gas potential of three different substrates, two algaes Saccharina latissima and Laminaria digitata and biosludge mixed with paper fiber was studied. This was done by batch experiments in a laboratory environment to examine the gas production and composition of the produced gas.Biogas production is a complex anaerobic digestion process in which various microorganisms decompose the substrate in steps and at the end produce biogas and a residue. Many factors affect the production of gas, for example the substrate content, temperature and pH in the digester.The analysis of methane potential were divided into two experiments. In experiment 1 substrates were digested along with inoculum from Växjö waste water treatment plant in a temperature of about 37 ?C.

Saproxylic lichens in managed forests have less substrate available than in unmanaged forests due to the shortage of dead wood. However, a Suitable substrate for these lichens could be dead branches on living trees. To this date, there has not been any systematically collected data about theabundance of dead branches on living trees and the lichens growing on these branches in managed forests. The aim of this study was to see where dead branches were located on living trees of Pinus sylvestris and Picea abies, how large the surface area of these branches was and what lichens grewof wood on these branches. The study was made in Finspång, Sweden, where 11 forest stands of P.sylvestris and P.

The global biodiversity loss is mainly due to human activities such as an intensification of forestry. Boreal forest ecosystems in Fennoscandia are characterized by disturbances such as forest fires, storms and floods. However, industrial forestry practices suppress forest fires and change the landscape, leading to a loss of habitats and associated species. Particularly lichen species with slow adaptation abilities and a strong substrate specificity face an extinction debt in boreal forests since their substrates are long-lived but no longer created. In this study the species-substrate relationship of two red-listed forest-fire dependent lichen species Carbon-icola anthracophila and C.

Treatment wetlands been showed efficient for reducing pollutant in waste water. In Lajeado ? RS, Brazil the landfill has poor leachate water treatment. It is necessary to supplement the treatment plant because they need to reduce BOD, COD and nitrate of the water going to recipient. A subsurface flow wetland (SSF) can be a good choice.  In order to be able to design an efficient SSF it is important to understand how the grain sizes of a substrate affect the reducing of pollutants in waste water.

The increasing threat of climate change has led to a increased need for models to predict future climat. In these models the changes in the soil carbon pools due to changes in microbiel degradation of the organic matter can lead to both positive and negative feedback-effects. There is however a lack of consensues on the temperature respons on degradation soil organic matter. This paper aim to give a better understanding on the factors controlling the temperature respons. The factors studied were the carbon substrate qualitity and the physiological status of the microorganisms.

The freshwater pearl mussel Margaritifera margaritifera is an endangered species. To survive, the young mussels demand a fairly coarse bottom material containing an appropriate quantity of organic substrate to survive without suffocating. In order to estimate if a bottom is suitable for juvenile mussels, samples of the sediment are needed. To obtain samples a sampling device called the Mulv collector has been developed within this project. Bottom samples were collected from 18 stations in 4 south Swedish streams, Vramsån, Kling-storpsbäcken, Brönnestadsån and Bräkneån.

One of Sweden?s sixteen national environmental objectives strives to decrease the impact on the climate. By 2020, green house gas emissions should be 40 % less compared to the levels of 1990 and a minimum of 50 % of the energy consumption should come from renewable energy sources. Because of this there is a great need of increasing the production of renewable energy. This is where biogas comes in as a competitive alternative.

The popularity of vegetated, or green roofs, has grown over the past years due to their potential function of maintaining urban environment quality. The aim of this study was to investigate if substrates with different organic material, peat and charcoal, with two contents (3 or 6 weight%) influenced the growth of the cuttings during the first critical period of the establishment. Biochar is used as amendment to agricultural soils and has, theoretically, many of the properties suitable as organic content in green roof-substrates. Peat is one of the materials that is commercially used in green roof industry. The substrate has been treated with different amounts of water.

The biogas plant Kungsängens gård, owned by Uppsala Vatten och Avfall AB, produces biogas and biomanure from organic household waste, food processing waste and slaughterhouse waste. In year 2012, 4.4 million Nm3 of biogas were produced from 25 200 tons of waste. Before digestion all substrate is sanitized by pasteurization at 70°C in order to kill pathogens. Another method, integrated thermophilic sanitation (ITS), is of interest in order to decrease the energy demand. The method implies that the substrate is sanitized during ten hours in the digestion chamber, where the temperature is 52°C.

The biogas plant Kungsängens gård, owned by Uppsala Vatten och Avfall AB, produces biogas and biomanure from organic household waste, food processing waste and slaughterhouse waste. In year 2012, 4.4 million Nm3 of biogas were produced from 25 200 tons of waste. Before digestion all substrate is sanitized by one hour?s pasteurization at 70°C in order to kill pathogens. Another method, integrated thermophilic sanitation (ITS), is of interest in order to decrease the energy demand.

One of the most serious and economically important defects caused by clostridia in milk products is the late blowing of semi-hard cheeses.Clostridia occur naturally in soil and can contaminate milk through crops contaminated by dung and soil followed by a less successful silage process, that give them opportunity to grow unaerobically. When anaerobic conditions occur, such as storage of semi-hard cheese, they ferment lactic acid to butyric acid and the gases CO2 and H2.At the fusion of Arla and MD Foods, a series of changes were conducted on the MPN method for lactic acid fermentation for clostridia in milk. These changes resulted in an increased accuracy due to an increased number of test tubes and the change of media from MRCM to BBB, Bryant & Burkey Broth, that was thought to be more selective for Cl. tyrobutyricum, the organism mostly found in hard cheese. When the number of dairy farmers that were given quality reduction fines increased, the new method was suspected and a validation was conducted.The validation included inoculation of different clostridia and bacillus strains into BBB substrate and enzymatic testing of positive samples with Rapid ID 32A.

Until 2020, 49% of Sweden's use of energy must derive from renewable sources. Greenhouse gases must as well decline with 40% compared to 1990 as a part of Sweden's 16 environmental objectives. Biogas is part of the effort to achieve these objectives, but as the demand for biogas increases, more substrates are needed to meet the demand. The purpose is to investigate the conditions for pea residue as substrate by performing lab-scale mesophilic digestion with different fractions and notch lengths and then to summarize pea residue as a substrate for biogas production. During anaerobic digestion, 1000 ml bottles were used as reactors for digestion.